AdapterRemoval v3.0.0-alpha3

Program #

Date	2025-05-19T19:27:18+0200
Command	adapterremoval3 --in-file1 SRR651031_1.fastq.gz --in-file2 SRR651031_2.fastq.gz --out-prefix example --merge --min-complexity 0.3 --min-mean-quality 20 --threads 8
Version	v3.0.0-alpha3
Runtime	19.2 seconds

Input #

	#Reads	#Bases	Read length	Q20	Q30	GC	N
Summary	49.4 M	4.45 G	90 bp	96.6 %	91.0 %	46.7 %	0.1 %
File 1	24.7 M	2.22 G	90 bp	97.3 %	92.0 %	46.7 %	0.0 %
File 2	24.7 M	2.22 G	90 bp	95.8 %	90.1 %	46.6 %	0.1 %

Base composition statistics/plots are based on 4.94 M (10.0 %) of input reads sampled during execution.

Output #

	#Reads	#Bases	Read length	Q20	Q30	GC	N
Passed*	36.8 M	3.81 G	103.6 bp	98.5 %	93.7 %	46.6 %	0.0 %
File 1	12.2 M	1.09 G	88.8 bp	98.7 %	93.4 %	47.0 %	0.0 %
File 2	12.2 M	1.07 G	87.7 bp	97.9 %	91.6 %	46.9 %	0.0 %
Merged	11.7 M	1.60 G	136.7 bp	98.9 %	95.4 %	46.2 %	0.0 %
Singleton	604 K	52.3 M	86.6 bp	97.4 %	90.6 %	46.0 %	0.0 %
Discarded*	801 K	65.8 M	82.1 bp	97.6 %	91.7 %	40.3 %	0.0 %

Base composition statistics/plots are based on 3.68 M (7.6 %) of output reads sampled during execution.

* The Passed column includes all read types except for Discarded reads.

Process summary #

Stage	Step		Reads	(%)	Bases	(%)	Mean Bases
Input	Raw reads	-	49.4 M	100.0 %	4.45 G	100.0 %	90.0 bp
Processing	Adapters	-	442 K	0.9 %	4.92 M	0.1 %	11.1 bp
	Merging	-	23.7 M	48.0 %	493 M	11.1 %	20.8 bp
	Low quality bases	-	3.33 M	6.7 %	73.6 M	1.7 %	22.1 bp
Filtering	Short reads	-	85.9 K	0.2 %	396 K	0.0 %	4.6 bp
	Mean quality	-	1.32 K	0.0 %	51.1 K	0.0 %	38.8 bp
	Low complexity reads	-	886 K	1.8 %	65.3 M	1.5 %	73.7 bp
Output	Filtered reads	-	48.4 M	98.0 %	3.81 G	85.7 %	78.6 bp

3 of 7 processing steps enabled, 3 of 5 filtering steps enabled. Numbers of reads are given in terms of input reads, meaning that a merged read counts for two. For Processing and Filtering, the numbers of bases specify how many were lost during each step.

Position quality distribution #

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Quality score distribution #

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Insert-size distribution #

Position quality distribution #

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