adapterremoval3 3.0.0

Summary #

Program #

Date 2026-04-12 18:00:30 CEST
Command adapterremoval3 --in-file1 SRR651031_1.fastq.gz --in-file2 SRR651031_2.fastq.gz --out-prefix example --merge --min-complexity 0.3 --min-mean-quality 20 --threads 8
Version 3.0.0-9765e930
Runtime 21.4 seconds

Input #

#Reads #Bases Read length Q20 Q30 GC N
Summary 49.4 M 4.45 G 90 bp 96.6 % 91.1 % 46.6 % 0.0 %
File 1 24.7 M 2.22 G 90 bp 97.3 % 92.0 % 46.7 % 0.0 %
File 2 24.7 M 2.22 G 90 bp 95.8 % 90.1 % 46.6 % 0.1 %

Base composition statistics/plots are based on 4.94 M (10.0 %) of input reads sampled during execution.

Output #

#Reads #Bases Read length Q20 Q30 GC N
Passed* 37.4 M 3.87 G 103.5 bp 98.5 % 93.7 % 46.5 % 0.0 %
File 1 12.7 M 1.13 G 88.7 bp 98.6 % 93.4 % 46.9 % 0.0 %
File 2 12.7 M 1.11 G 87.6 bp 97.8 % 91.6 % 46.8 % 0.0 %
Merged 11.9 M 1.62 G 136.7 bp 98.9 % 95.4 % 46.1 % 0.0 %
Singleton 132 K 10.9 M 82.4 bp 95.3 % 85.3 % 46.9 % 0.0 %
Discarded* 134 K 1.70 M 12.7 bp 90.5 % 80.3 % 22.6 % 0.0 %

Base composition statistics/plots are based on 3.74 M (7.6 %) of output reads sampled during execution.

* The Passed column includes all read types except for Discarded reads.

Process summary #

Stage Step Reads (%) Bases (%) Mean Bases
Input Raw reads - 49.4 M 100.0 % 4.45 G 100.0 % 90.0 bp
Processing Adapters - 442 K 0.9 % 4.92 M 0.1 % 11.1 bp
Merging - 23.7 M 48.0 % 493 M 11.1 % 20.8 bp
Low quality bases - 3.33 M 6.7 % 73.7 M 1.7 % 22.1 bp
Filtering Short reads - 86.1 K 0.2 % 397 K 0.0 % 4.6 bp
Ambiguous bases - 190 0.0 % 7.03 K 0.0 % 37.0 bp
Mean quality - 1.25 K 0.0 % 49.0 K 0.0 % 39.1 bp
Low complexity reads - 46.4 K 0.1 % 1.25 M 0.0 % 26.9 bp
Output Filtered reads - 49.3 M 99.7 % 3.87 G 87.1 % 78.6 bp

3 of 7 processing steps enabled, 4 of 5 filtering steps enabled. Numbers of reads are given in terms of input reads, meaning that a merged read counts for two. For Processing and Filtering, the numbers of bases specify how many were lost during each step.

Input #

Position quality distribution #

Nucleotide content #

Quality score distribution #

GC Content #

Analyses #

Insert-size distribution #

Insert sizes inferred for 48.0 % of reads

Output #

Position quality distribution #

Nucleotide content #

Quality score distribution #

GC Content #

For comments, suggestions, or feedback, please use GitHub Issues. If you use AdapterRemoval, please cite Schubert et. al. 2016:

Schubert, Lindgreen, and Orlando (2016). AdapterRemoval v2: rapid adapter trimming, identification, and read merging. BMC Research Notes, 12;9(1):88.

This report was generated using Pure (license) and Vega-Lite (license).